The use of antibiotics is not permitted in Switzerland; moreover, no other medication exists to combat European foul brood. Hives with symptoms must therefore be destroyed in order to limit the outbreak, and the contaminated material must be sanitised. Since prevention is the best cure, early detection is desirable. Numerous studies have been undertaken and documents have been created at the Swiss Bee Research Centre and elsewhere to draw the attention of beekeepers to these problems and inform them about the causes and the control measures to be implemented.
Once upon a time, the VANA story based on the review "virion-associated nucleic acid-based metagenomics: A decade of advances in molecular characterization of plant viruses".
Moubset O., François S., Maclot F., Palanga E., Julian C., Claude L., Fernandez E., Rott P., Daugrois J-H., Antoine-Lorquin A., Bernardo P., Blouin A., Temple C., Kraberger S., Fontenele R., Harkins GW., Ma X., Marais A., Candresse T., Ben Chehida S., Lefeuvre P., Lett JM., Varsani A., Ogliastro M., Martin DM., Filloux D., Roumagnac P.
Once upon a time, the VANA story based on the review "virion-associated nucleic acid-based metagenomics: A decade of advances in molecular characterization of plant viruses".
In: 19e Rencontres de virologie végétale (RVV2023). 15 January, Aussois (FR). 2023.
Over the last decade, viral metagenomic studies have resulted in the discovery of thousands of previously unknown viruses. These studies are likely to play a pivotal role in obtaining an accurate and robust understanding of how viruses affect the stability and productivity of ecosystems. Among the metagenomics-based approaches that have been developed since the beginning of the 21st century, shotgun metagenomics applied specifically to virion-associated nucleic acids (VANA) has been used to disentangle the diversity of the viral world. We summarize herein the results of 25 VANA-based studies, focusing on plant and insect samples conducted over the last decade (2010 to 2022). Collectively, viruses from 94 different families were reliably detected in these studies, including capsidless RNA viruses that replicate in fungi, oomycetes, and plants. Finally, strengths and weaknesses of the VANA approach are summarized and perspectives of applications in detection, epidemiological surveillance, environmental monitoring, and ecology of plant viruses are provided.
