Control

The use of antibiotics is not permitted in Switzerland; moreover, no other medication exists to combat European foul brood. Hives with symptoms must therefore be destroyed in order to limit the outbreak, and the contaminated material must be sanitised. Since prevention is the best cure, early detection is desirable. Numerous studies have been undertaken and documents have been created at the Swiss Bee Research Centre and elsewhere to draw the attention of beekeepers to these problems and inform them about the causes and the control measures to be implemented.

Merkblatt zur erkennung von Bienenbrutkrankheiten (German (PDF, 357 kB, 18.10.2017) and French (PDF, 226 kB, 02.02.2017))
M. Tschumi (2011)

Interlaboratory validation trial report on multiplex real-time PCR method for molecular serotyping and identification of the 30 major clonal complexes of Listeria monocytogenes circulating in food in Europe.

Institutional Repository Agroscope

Capitaine K., Te S., Asséré A., Plodková H., Michel V., Sabrou P., Bourdonnais E., Gillot G., Mouhali N., Brauge T., Dumaire C., Feurer C., Marti Serrano E.

Interlaboratory validation trial report on multiplex real-time PCR method for molecular serotyping and identification of the 30 major clonal complexes of Listeria monocytogenes circulating in food in Europe.

Microbiology Spectrum, 13, (7), 2025.

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Riassunto:

The performance of a new method developed in 2021 by the European Union Reference Laboratory (EURL) for Listeria monocytogenes based on 12 multiplex real-time PCR, allowing the identification of the molecular serotype and the 30 major L. monocytogenes multilocus sequence typing clonal complexes (CC), was assessed through a European interlaboratory validation trial (ILVT). This ILVT was adapted from ISO standard 16140 part 6. Overall, 98 blinded pure strains of Listeria (monocytogenes or spp.), previously characterized by the EURL, were sent to 15 laboratories distributed in 11 countries. The molecular serotype had to be identified for 20 strains of the ILVT panel, while CC identification had to be performed for the whole panel. The results of the 12 multiplex real-time PCR were reproducible between the participating laboratories with high individual concordance values for molecular serotyping (100%) and CC identification (90.8%–100%) irrespective of DNA extraction protocols, PCR master mixes, and thermocycler diversity. Master mixes identified as incompatible with some of the multiplex real-time PCR were excluded from the method. The overall concordance of the results was sufficient for the method to be confidently applied in other laboratories involved in L. monocytogenes typing.

ISSN Print: 2165-0497
ISSN Online: 2165-0497
Digital Object Identifier (DOI): https://doi.org/10.1128/spectrum.00116-25
ID pubblicazione (Codice web): 59906 Inviare via e-mail

Inizio pagina

Tierseuchenverordnung - Abschnitt: Sauerbrut der Bienen (German, French and Italian)

Technische Weisungen des BLV - Sauerbrut (German, French and Italian)

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Interlaboratory validation trial report on multiplex real-time PCR method for molecular serotyping and identification of the 30 major clonal complexes of Listeria monocytogenes circulating in food in Europe.