In their environments (such as soil, rhizospheres, plants, mucosal membranes and guts), fungi are part of a microbial community where they constantly are in interaction. These interactions activate silent genes that are not normally active under classical growth condition in laboratories. These genes are particularly related to metabolite production such as pheromones, defence molecules and metabolites of symbiotic associations [1]. This induction phenomenon was recently illustrated to be a general consequence of fungal co-culture with a large panel of fungal interaction tested [2, 3]. To explore in details the resulting fungal metabolome modifications, the design of the experiment need to be devised to meet high analytical and biological reproducibility. In order to tackle this issue a high throughput UHPLC-TOF-MS based metabolomic approach has been developed for the screening of miniaturized 12-wellplates solid fungal co-cultures, mimicking standard petri dishes growth conditions. The strategy provided reproducibility in accordance to metabolomic standards [4]. It highlighted the induction of many metabolites. And the time series used enabled to assess typical metabolites induction dynamic patterns during the interaction between Aspergillus clavatus and Fusarium sp. Acknowledgements: This work was supported by Swiss National Science Foundation Sinergia Grant CRSII3_127187 (to J.-L. W., K. G. and M. M.) Références bibliographiques : [1] K. Scherlach, C. Hertweck, Org. Biomol. Chem., 7 (2009) 1753-1760. [2] S. Bertrand, O. Schumpp, N. Bohni, A. Bujard, A. Azzollini, M. Monod, K. Gindro, J.-L. Wolfender, J. Chromatogr., A, (2013) DOI: 10.1016/j.chroma.2013.1001.1098. [3] G. Glauser, K. Gindro, J. Fringeli, J.-P. De Joffrey, S. Rudaz, J.-L. Wolfender, J. Agric. Food Chem., 57 (2009) 1127-1134. [4] E.J. Want, I.D. Wilson, H. Gika, G. Theodoridis, R.S. Plumb, J. Shockcor, E. Holmes, J.K. Nicholson, Nat. Protoc., 5 (2010) 1005-1018. Mots-clés : Aspergillus clavatus, Fungal Interactions, Fusarium, Solid Media Co-Culture, UHPLC-TOF-MS Metabolomics
