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Biolley C., Tretola M., Bee G., Jud Khan C., Silacci P.

Punicalagin increases glutamate absorption in diﬀerentiated Caco-2 cells by a mechanism involving gene expression regulation of an EAAT3 transporter.

Food & Function, 10, (9), 2019, 5333-5338.

This study investigates, for the ﬁrst time, the ability of punicalagin to modulate intestinal glutamate uptake by upregulation of the expression of one of its transporters present on the enterocyte membrane. The use of an Ussing chamber revealed an increase in glutamate transport in diﬀerentiated Caco-2 cells after punicalagin treatment for 24 h. This cell line constitutively expresses two glutamate transporters: EAAT1 and EAAT3. In response to punicalagin, the expression of EAAT3 was increased, at both mRNA and protein levels, but not that of EAAT1. Transfection with EAAT3-targeting siRNA speciﬁcally altered basal and induced EAAT3 gene expression, decreasing the positive eﬀect of punicalagin on glutamate uptake. These data conﬁrmed the involvement of EAAT3 in increasing glutamate uptake by enterocytes after punicalagin treatment.

ISSN Print: 2042-6496
ISSN Online: 2042-650X
Digital Object Identifier (DOI): https://doi.org/10.1039/c9fo00191c
Publikations-ID (Webcode): 42203 Per E-Mail versenden

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Punicalagin increases glutamate absorption in diﬀerentiated Caco-2 cells by a mechanism involving gene expression regulation of an EAAT3 transporter.

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Punicalagin increases glutamate absorption in diﬀerentiated Caco-2 cells by a mechanism involving gene expression regulation of an EAAT3 transporter.