In the 1990s, the Bee Research Centre took part in a screening programme testing the potential effectiveness of over 100 essential oils and essential oil components against the varroa mite. Although sage, hyssop and thyme oils proved interesting, only thymol has been used in acaricide products. Thymol treatments require little effort, and there are a variety of preparations on the market. The advantages and drawbacks of thymol use are listed in the articles "Apilife VAR: A new varroacide with thymol as the main ingredient" and "THYMOVAR for Varroa control".
Detection of single nucleotide polymorphisms in virus genomes assembled from high-throughput sequencing data: Large-scale performance testing of sequence analysis strategies.
Rollin J., Bester R., Brostaux Y., Caglayan K., De Jonghe K., Eichmeier A., Foucart Y., Haegeman A., Koloniuk I., Kominek P., Maree H., Onder S., Posada Céspedes S., Roumi V., Šafářová D., Schumpp O., Ulubas Serce C., Sõmera M., Tamisier L., Vainio E., van der Vlugt R. A. A., Massart S.
Detection of single nucleotide polymorphisms in virus genomes assembled from high-throughput sequencing data: Large-scale performance testing of sequence analysis strategies.
Recent developments in high-throughput sequencing (HTS) technologies and bioinformatics have drastically changed research in virology, especially for virus discovery. Indeed, proper monitoring of the viral population requires information on the different isolates circulating in the studied area. For this purpose, HTS has greatly facilitated the sequencing of new genomes of detected viruses and their comparison. However, bioinformatics analyses allowing reconstruction of genome sequences and detection of single nucleotide polymorphisms (SNPs) can potentially create bias and has not been widely addressed so far. Therefore, more knowledge is required on the limitations of predicting SNPs based on HTS-generated sequence samples. To address this issue, we compared the ability of 14 plant virology laboratories, each employing a different bioinformatics pipeline, to detect 21 variants of pepino mosaic virus (PepMV) in three samples through large-scale performance testing (PT) using three artificially designed datasets. To evaluate the impact of bioinformatics analyses, they were divided into three key steps: reads pre-processing, virus-isolate identification, and variant calling. Each step was evaluated independently through an original, PT design including discussion and validation between participants at each step. Overall, this work underlines key parameters influencing SNPs detection and proposes recommendations for reliable variant calling for plant viruses. The identification of the closest reference, mapping parameters and manual validation of the detection were recognized as the most impactful analysis steps for the success of the SNPs detections. Strategies to improve the prediction of SNPs are also discussed.
Although the use of thymol leads to residues in the wax and honey, these are negligible from a toxicological viewpoint. Since about 2008 the use of thymol-based products has been declining in Switzerland, and beekeepers making use of these products have tended to experience greater winter losses than those using e.g. formic acid.
