Nutrichip: an integrated microfluidic system for in vitro investigation of the immunemodulatory function of dairy products.
Dans: Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS). October 2-6, Seattle, Washington, USA. 2011, 650-652.
We introduce the concept of an artificial human gastro-intestinal tract (GIT) for investigating the potential of immunmodu-latory function of milk. More specifically, we are investigating the effect of milk on immune cells activation by creating con-ditions close to those of the human GIT, employing an in vitro model of GIT epithelial cells (Caco2) in co-culture with im-mune cells (THP1) on a Transwell. The cytokines (IL1/6) and Toll-like receptors (TLR2/4) expressions by the immune cells are monitored upon the application of milk on the epithelial cell layer. We also present initial results of a Lab-on-a-Chip-based in vitro GIT model (the NutriChip).
