Regenerative agriculture has gained attention in mainstream media, academic literature, and international politics in recent years. While many practices and outcomes relate to RA, there is no uniform definition of the term, and only a few comprehensive scientific studies exist of “real-life” farms and the complexity of what is considered regenerative management and its impact on soil health. This study aimed to relate the impact of single and various combinations of regenerative management practices to soil health indicators on Gotland, Sweden. Soil health of 17 farm fields and six gardens was assessed on 11 farms that had applied regenerative agricultural practices for zero to 30 years. We measured a variety of physical (bulk density, infiltration rate, wet aggregate stability, root depth and abundance, penetration resistance), chemical (pH, electric conductivity, C:N ratio, total organic carbon) and biological (earthworm abundance, active carbon, microbial biomass carbon) soil indicators. These parameters were related to regenerative practices (reduced tillage, application of organic matter, livestock integration, crop diversity, and share of legumes and perennials) through a combination of hierarchical clustering, Analysis of Variance and Tukey's tests, principal component analysis, and multiple linear regressions. At our study sites, the application of organic matter had a positive impact on bulk density, carbon-related parameters, wet aggregate stability, and infiltration rate, while reduced tillage and increased share of perennials combined had a positive impact on vegetation density, root abundance and depth, and wet aggregate stability. The field plots were divided into four clusters according to their management, and we found significantly higher values of total organic carbon (*), C:N (*), infiltration rate (**), and earthworm abundance (*) for crop-high-org-input, the management cluster with highest values of organic matter application and no tillage. We found significantly higher values of vegetation density (***) and root abundance (**) for perm-cover-livestock, the cluster with no tillage, integration of livestocks, and permanent cover (*** p < 0.001, ** p < 0.01, *p < 0.05, °p < 0.1). We support existing knowledge on positive impacts of regenerative practices, namely, the addition of organic amendment that improved carbon-related parameters, as well as the positive effects on soil structure of reduced tillage in combination with an increased share of perennials. We argue for an outcome-based, and principle-led concept of regenerative agriculture as a context-dependent agricultural approach.
