Aims : The aims of this study were to investigate the physiological behavior (plant hydraulics, gas exchange) of the cultivar Pinot Noir in the field under progressively increasing conditions of water stress and analyze the effects of drought on grape and wine quality. Methods and results : Grapevines of the variety Vitis vinifera L. cv. Pinot noir (clone 9-18, grafted onto 5BB) were subjected to different water regimes (irrigation treatments) over the growing season. Physiological indicators were used to monitor plant water status (leaf and stem water potentials and relative carbon isotope composition (δ13C) in must sugars). Leaf gas exchange (net photosynthesis A and transpiration E), leaf stomatal conductance (gs), specific hydraulic conductivity in petioles (Kpetiole), yield components, berry composition at harvest, and organoleptic quality of wines were analyzed over a 7-year period, between 2009 and 2015, under relatively dry conditions in the canton of Wallis, Switzerland. A progressively increasing water deficit, observed throughout the season, reduced the leaf gas exchange (A and E) and gs in non-irrigated vines. The intrinsic water use efficiency (WUEi, A/gs) increased during the growing season and was greater in water-stressed vines than in well-watered vines (irrigated vines). This rise in WUEi was correlated with an increase in δ13C in must sugars at harvest. Drought led to decreases in Kpetiole, E and sap flow in stems. A decrease in vine plant vigor was observed in vines that had been subjected to water deficits year after year. Moderate water stress during ripening favored sugar accumulation in berries and caused a reduction in total acidic and malic contents in must and available nitrogen content (YAN). Wines produced from water-stressed vines had a deeper color and were richer in anthocyanins and phenol compounds compared with wines from well-watered vines with no water stress. The vine water status greatly influenced the organoleptic quality of the resulting wines. Wines made from non-irrigated vines with a water deficit presented more structure and higher-quality tannins. They were also judged to be more full-bodied and with blended tannins than those made from irrigated vines. Conclusions : Grape ripening and resulting Pinot Noir wines were found to be largely dependent on the water supply conditions of the vines during the growing season, which influenced gas exchange and plant hydraulics. Significance and impact of the study : Plant water status constitutes a key factor in leaf gas exchange, canopy water use efficiency, berry composition and wine quality.
