Koloniuk I., Thekke-Veetil T., Reynard J.-S., Mavriˇc Pleško I., Pˇribylová J., Brodard J., Kellenberger I., Sarkisova T., Špak J., Lamovšek J., Massart S., Ho T., Postman J. D., Tzanetakis I. E.
Molecular characterization of divergent closterovirus isolates infecting ribes species.
Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.
