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Small bias of one highly dominant taxon on community analyses using amplicon sequencing.

Journal of Microbiological Methods, 178, 2020.

Amplicon sequencing allows for simultaneous assessment of inoculation success and responses of indigenous communities after microbial inoculation. However, the presence of a highly dominant taxon may bias correct assessment of communities due to dilution effects, chimera formation, or preferential amplification. Here, we present a simple test to assess these biases.

ISSN Print: 0167-7012
ISSN Online: 0167-7012
Digital Object Identifier (DOI): https://doi.org/10.1016/j.mimet.2020.106069
Publication-ID (Web Code): 45424 Sending by e-mail

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Small bias of one highly dominant taxon on community analyses using amplicon sequencing.

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Small bias of one highly dominant taxon on community analyses using amplicon sequencing.