Amplicon sequencing allows for simultaneous assessment of inoculation success and responses of indigenous communities after microbial inoculation. However, the presence of a highly dominant taxon may bias correct assessment of communities due to dilution effects, chimera formation, or preferential amplification. Here, we present a simple test to assess these biases.
Mayerhofer J., Enkerli J., Widmer F.
Small bias of one highly dominant taxon on community analyses using amplicon sequencing.
Journal of Microbiological Methods, 178, 2020.
ISSN Print: 0167-7012
ISSN Online: 0167-7012
Digital Object Identifier (DOI): https://doi.org/10.1016/j.mimet.2020.106069
Publikations-ID (Webcode): 45424 Per E-Mail versenden
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