The mechanisms behind thiol precursor accumulation in grapes remain incompletely understood, nor are the ways in which they can be improved by agronomic practices. A six-year field trial studied the physiological response of the Swiss white cultivar Vitis vinifera Arvine, rich in varietal thiols and precursors, to canopy management, i.e. leaf removal and canopy height.. Five treatments were set up in a randomized block design to assess the impacts of 1) pre-flowering LR (i.e. pre-flowering or full-flowering stages) and 2) compensating for the leaf area removed in the cluster zone by increasing the trimming height (i.e. 100 or 150 cm canopy height), compared with a non-defoliated control treatment. Intensive pre-flowering LR severely reduced yield potential (–47% on average) and reduced the concentration of 3-mercaptohexanol precursors (P-3MH) in the must (–21%; p-value < 0.10). Decreasing earliness modulated the impact of LR on yield (–12%) and P-3MH concentration (–6%). Compensating for suppressed leaf area by increasing the trimming height slightly enhanced grape ripening (+1% total sugars; –3% titratable acidity), slightly improved the overall quality of the wine (color intensity, volume), while having no impact on must P-3MH concentration or on wine bouquet. Observing the long-term impact of each LR treatment separately provided insights into the physiological mechanisms influencing fruit development and aroma formation. This trial is part of a larger project on canopy management and its impact on grape composition in temperate Swiss climatic conditions.