For a pilot study on germplasm collections, leaves were collected from six apple trees (Malus domestica) numbered Q9, Q27, Q35, Q37, Q39 and Q41 in a Belgian experimental orchard (CRA-W) in June 2019 and June 2020. At the time of sampling and during subsequent visits, no viral symptoms were observed on the trees. However, Apple luteovirus 1 (ALV-1) has been identified in trees with Rapid apple decline, Apple rubodvirus 1 (ARWV-1) is associated with apple rubbery wood disease, and Apple hammerhead viroid (AHVd) infection presents variable symptoms including swelling or limb flattening.