The present trial follows a previous study about pre-flowering leaf removal (LR) (Verdenal et al., 2019) and validates the sustainability of mechanical pre-flowering LR under local Swiss vineyard conditions, using a low-pressure double airflow, to reduce the cost of laborious bunch thinning. In previous studies, pre-flowering LR has shown additional benefits over post-berry-set LR in terms of yield regulation and grape and wine compositions. This trial had two objectives: 1) to test the technical feasibility of mechanical pre-flowering LR, using a low-pressure double airflow and 2) to observe the impact of this practice over five years on yield parameters, grape composition at harvest and wine quality over five years. For this purpose, a trial was conducted on the two cultivars, Doral (white) and Gamay (red), to compare four LR treatments, that is, A) mechanical post-berry-set LR, B) manual pre-flowering LR, C) mechanical pre-flowering LR, and D) double mechanical pre-flowering + post-berry-set LR. More broadly, this work provides practical insights into the consequences of pre-flowering LR on the grapevine, pointing out the advantages and the limits of intensity, timing and mechanisation of this practice. In comparison with the mechanical post-berry-set LR, mechanical pre-flowering LR induced a 7 % loss in bud fruitfulness, a 30 % yield loss and a 3 % gain in total soluble solids (TSS) accumulation in grapes in both cultivars, although the improvement in grape maturity was small and uneven through the years; Gamay anthocyanin concentration remained stable. Pre-flowering LR had no overall impact on the quality of Doral and Gamay wines. Damage was observed on the inflorescences due to the intensity of mechanical pre-flowering LR, which resulted in fewer berries per cluster and a lower yield than with manual LR by the same date. A second LR after berry set was also tested to limit the growth of laterals and clean the clusters from the remaining flower caps to prevent the development of fungal diseases, but it did not show any benefit over a single pre-flowering LR. In conclusion of this trial, a single, moderate mechanical pre-flowering LR is an effective and sustainable practice under temperate climatic conditions, to reduce the costs of laborious manual pre-flowering LR.
